PhD- experimental investigation of self-assembly and phase transitions in lipids/nucleic acids mixtures

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About ESPCI Paris - PSL :
ESPCI (https://www.espci.fr/en/) is a leading French “Grande Ecole” founded in 1882, educating undergraduate and graduate students through a programme merging basic science and engineering, as well as a world-renowned research institution. ESPCI Paris hosts 11 research units, all associated to CNRS and/or INSERM and/or other Parisian Universities in the form of joined research units, covering the fields of physics, chemistry and biology. ESPCI Paris has setup a tradition of excellence in research, with distinguished scientists who have contributed to its history. Five Nobel Prize awardees are a testimony of the culture of excellence in the Institute. Favoring interdisciplinary and operating at the frontiers between fundamental research and innovation, are two major objectives of the School.
ESPCI is also a member of “Paris Science et Lettres” Research University (https://www.univ-psl.fr/en), an ambitious collaboration project between 26 research institutions in a broad range of disciplines from engineering, chemistry and oncology to economics, management, humanities and performing arts.

About the laboratory/Research Unit  :
The position will be based in the Laboratoire de Biochimie (LBC) which is part of the UMR8231 Chimie, Biologie, Innovation at ESPCI Paris/CNRS. Research at LBC is focused on droplet-based microfluidics for applications in biology and in fundamental investigations in origin of life.

Job environment :
Institut Pierre-Gilles de Gennes, ESPCI Paris, PSL ResearchUniversityhttps://www.institut-pgg.com
6 rue Jean Calvin, 75005, Paris, France

Job description :
The PhD position isfunded by the Ecole doctorale 388 - Chimie Physique et Chimie Analytique de Paris-Centre. The aim of this research consists in studying the formation of ordered phases typical of liquid crystals in mixtures of lipids and nucleic acids monomers and oligomers (DNA and RNA) to investigate the emergence of effective proto-cellular structures and nucleic acids non-enzymatic polymerization. This project foresees the realization of a microfluidic open reactor where mixtures of monomeric building blocks are submitted to hydration-dehydration cycles, where polymerization takes place in the dry phase by condensation and compartmentalization takes place in the wet phase by vesicles formation. The final goal is to probe the evolution of the starting RNA population trough cycles.

Experimental techniques :
Phases characterization by microscopy (polarized, fluorescence, confocal, FRAP, FRET), X-ray scattering, UV-Vis spectroscopy ; Products analysis : PAGE, enzymatic essay, HPLC, MALDI, LC-MS, NMR ; Lithography and soft-lithography techniques for PDMS chip realization.

Profile of the applicant

Skills required :
Experimentalist with a strong background in organic chemistry, supramolecular chemistry, biochemistry, or soft-metter physics. Experience in microscopy, experimental investigation of lipid/nucleic acids solutions (samples preparation and characterization) and interest in prebiotic chemistry will be a plus. He/she will have good proficiency in oral and written English, collaborative skills and ability to communicate.

Education level :
Applicants must have a diploma (or obtain a diploma before their employment starts) granting access to doctoral studies (typically a MSc degree). Candidates may apply prior to obtaining their master’s degree but cannot begin before they receive it.

Experience required :
A practical experience in good laboratory practices and experimentation will be highly considered

Contacts :
Philippe NGHE : Maître de Conférences at ESPCI ;https://blog.espci.fr/nghe/philippe.nghe@espci.psl.eu
Tommaso P. FRACCIA : Junior Researcher at ESPCI ;tommaso.fraccia (arobase) espci.fr" class='spip_url spip_out auto' rel='nofollow external'>https://blog.espci.fr/tfraccia/tommaso.fraccia@espci.fr

Application procedure :
Send a CV and a motivation letter to tommaso.fraccia (arobase) espci.fr to introduce yourself, describe your background and previous experiences of relevance for the position, and present your future career focus.

Deadline for applications :16 May 2021

Employment indicative starting date:September 2021

Access
 :
Métro line 7 (Place Monge/CensierDaubenton) - RER B (Luxembourg) - Bus 21, 27 & 47 - 3 Vélib’ stations





ÉCOLE SUPÉRIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS
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